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Reprod Sci. 2015 Apr;22(4):452-61. doi: 10.1177/1933719114542009. Epub 2014 Jul 18.

The role of SPRASA in female fertility.

Author information

1
Department of Obstetrics and Gynaecology, Faculty of Medical and Health Sciences, School of Medicine, The University of Auckland, Auckland, New Zealand.
2
Department of Obstetrics and Gynaecology, Faculty of Medical and Health Sciences, School of Medicine, The University of Auckland, Auckland, New Zealand olivia.holland@auckland.ac.nz.

Abstract

Fertility is a complex process and infertility can have many causes. Sperm protein reactive with antisperm antibody (SPRASA)/sperm lysozyme-like protein 1 is a protein discovered as the target of autoantibodies in infertile men and previously thought to be expressed only in sperm. Using a bovine in vitro fertilization model, we have shown that SPRASA antiserum reduced sperm binding to zona-free oocytes and the development of embryos to morulae but did not affect the postfertilization cleavage rate to 2 cells or sperm motility. We demonstrated that SPRASA was expressed in ovarian follicles, corpora lutea, and oocytes by a combination of reverse transcription-polymerase chain reaction and immunohistochemistry. Female mice immunized with SPRASA had profound infertility following timed matings and those mice that did become pregnant had reduced fetal viability. The levels of antibodies reactive with SPRASA in 204 fertile and 202 infertile couples were elevated in 3 infertile but no fertile women. Together, these results indicate that SPRASA has a role in female fertility.

KEYWORDS:

SLLP1; SPACA3; SPRASA; contraceptive; infertility; oocyte

PMID:
25038051
PMCID:
PMC4812687
DOI:
10.1177/1933719114542009
[Indexed for MEDLINE]
Free PMC Article

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