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J Am Soc Mass Spectrom. 2014 Oct;25(10):1730-8. doi: 10.1007/s13361-014-0956-2. Epub 2014 Jul 20.

ECD of tyrosine phosphorylation in a triple quadrupole mass spectrometer with a radio-frequency-free electromagnetostatic cell.

Author information

1
Department of Chemistry, Oregon State University, Corvallis, OR, 97333, USA, valery.voinov@orst.edu.

Abstract

A radio frequency-free electromagnetostatic (EMS) cell devised for electron-capture dissociation (ECD) of ions has been retrofitted into the collision-induced dissociation (CID) section of a triple quadrupole mass spectrometer to enable recording of ECD product-ion mass spectra and simultaneous recording of ECD-CID product-ion mass spectra. This modified instrument can be used to produce easily interpretable ECD and ECD-CID product-ion mass spectra of tyrosine-phosphorylated peptides that cover over 50% of their respective amino-acid sequences and readily identify their respective sites of phosphorylation. ECD fragmentation of doubly protonated, tyrosine-phosphorylated peptides, which was difficult to observe with FT-ICR instruments, occurs efficiently in the EMS cell.

PMID:
25037842
PMCID:
PMC4163116
DOI:
10.1007/s13361-014-0956-2
[Indexed for MEDLINE]
Free PMC Article

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