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Elife. 2014 Jul 17;3. pii: e03401. doi: 10.7554/eLife.03401.

Concerning RNA-guided gene drives for the alteration of wild populations.

Author information

1
Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, United States.
2
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, United States.

Abstract

Gene drives may be capable of addressing ecological problems by altering entire populations of wild organisms, but their use has remained largely theoretical due to technical constraints. Here we consider the potential for RNA-guided gene drives based on the CRISPR nuclease Cas9 to serve as a general method for spreading altered traits through wild populations over many generations. We detail likely capabilities, discuss limitations, and provide novel precautionary strategies to control the spread of gene drives and reverse genomic changes. The ability to edit populations of sexual species would offer substantial benefits to humanity and the environment. For example, RNA-guided gene drives could potentially prevent the spread of disease, support agriculture by reversing pesticide and herbicide resistance in insects and weeds, and control damaging invasive species. However, the possibility of unwanted ecological effects and near-certainty of spread across political borders demand careful assessment of each potential application. We call for thoughtful, inclusive, and well-informed public discussions to explore the responsible use of this currently theoretical technology.

KEYWORDS:

CRISPR; cas9; chromosomes; ecological engineering; ecology; emerging technology; gene drive; genes; none; population engineering

Conflict of interest statement

KME: Filed for a patent concerning RNA-guided gene drives. ALS: Filed for a patent concerning RNA-guided gene drives. GMC: Filed for a patent concerning RNA-guided gene drives. The other author declares that no competing interests exist.

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