Format

Send to

Choose Destination
J Radiat Res. 2014 Nov;55(6):1107-13. doi: 10.1093/jrr/rru067. Epub 2014 Jul 17.

Protective effect of inhalation of hydrogen gas on radiation-induced dermatitis and skin injury in rats.

Author information

1
Department of Radiology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
2
Division of Environmental Medicine, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan Second Division, Aeromedical Laboratory, Japan Air Self-Defense Force, 1-2-10 Sakae, Tachikawa, Tokyo 190-8585, Japan fujitama@ndmc.ac.jp bxb01424@nifty.com.
3
Division of Biomedical Engineering, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
4
Division of Environmental Medicine, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
5
Department of Health Crisis Management, National Institute of Public Health, 2-3-6 Minami, Wako, Saitama 351-0197, Japan.

Abstract

The effect of inhalation of hydrogen-containing gas (1.3% hydrogen + 20.8% oxygen + 77.9% nitrogen) (HCG) on radiation-induced dermatitis and on the healing of healing-impaired skin wounds in rats was examined using a rat model of radiation-induced skin injury. An X-ray dose of 20 Gy was irradiated onto the lower part of the back through two holes in a lead shield. Irradiation was performed before or after inhalation of HCG for 2 h. Inhalation of HCG significantly reduced the severity of radiodermatitis and accelerated healing-impaired wound repair. Staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) and 8-hydroxy-2(')-deoxyguanosine (8-OHdG) showed that the proportion of apoptotic keratinocytes and the level of staining in the X-irradiated skin of rats that pre-inhaled HCG were significantly lower than that of rats which did not pre-inhale HCG. Cutaneous full-thickness wounds were then created in the X-irradiated area to examine the time-course of wound healing. X-irradiation significantly increased the time required for wound healing, but the inhalation of HCG prior to the irradiation significantly decreased the delay in wound healing compared with the control and post-inhalation of HCG groups. Therefore, radiation-induced skin injury can potentially be alleviated by the pre-inhalation of HCG.

KEYWORDS:

antioxidant; healing-impaired wound; hydrogen; radiodermatitis; radioprotection

PMID:
25034733
PMCID:
PMC4229932
DOI:
10.1093/jrr/rru067
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center