Format

Send to

Choose Destination
Exp Mol Pathol. 2014 Oct;97(2):225-33. doi: 10.1016/j.yexmp.2014.07.007. Epub 2014 Jul 14.

Associated changes in the transcription levels of IL-17A and tight junction-associated genes in the duodenal mucosa of rhesus macaques repeatedly exposed to simian/human immunodeficiency virus.

Author information

1
National Center for AIDS/STD Control and Prevention, China-CDC, Beijing 102206, PR China.
2
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China.
3
National Center for AIDS/STD Control and Prevention, China-CDC, Beijing 102206, PR China. Electronic address: guibyang@public.bta.net.cn.

Abstract

BACKGROUND:

Mucosal barrier dysfunction might play a key role in HIV/AIDS, yet the early effects of HIV-1 on intestinal mucosal barrier, especially tight junctions (TJ) have not been well addressed.

AIMS:

To investigate the effects of acute HIV-1 infection on the expression of intestinal IL-17A and TJ-associated genes using an NHP-AIDS model.

METHODS:

TaqMan probe real-time RT-PCR methods were established and claudin-1, claudin-3, occludin and zonula occluden-1 (ZO-1) mRNA levels in the duodenal biopsies of rhesus macaques collected before and after rectal exposures to SHIV-SF162P4 were examined and compared with that of IL-17A, IL-6, TGF-β, RORγt, T-bet, Foxp-3 and GATA-3.

RESULTS:

The mRNA levels of TJ-associated genes were statistically significantly reduced soon after viral exposures and the mRNA levels of claudin-1, occludin and ZO-1 in viral positive tissues (from Group I) were lower than that in viral negative tissues (from Group II) after viral exposure. IL-17A mRNA levels were also decreased and positively correlated with the mRNA levels of the TJ-associated genes after viral exposure or infection, although the levels of IL-6, TGF-β and RORγt mRNA showed no statistical difference. The levels of GATA-3 mRNA in tissues collected before viral exposure were statistically different between Group I and Group II animals. The balance between T-bet and GATA-3 mRNA levels in Group II was markedly altered and statistically significantly different from that in Group I.

CONCLUSIONS:

Acute SHIV, and by extension HIV infection could affect the expression of TJ-associated genes, probably through IL-17A and other immune alterations.

KEYWORDS:

Chinese rhesus macaques; Claudins; IL-17A; Occludin; SHIV; Tight junction; Zonula occluden-1

PMID:
25034324
DOI:
10.1016/j.yexmp.2014.07.007
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center