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Bioconjug Chem. 2014 Aug 20;25(8):1395-401. doi: 10.1021/bc5002467. Epub 2014 Aug 1.

Homogeneous bispecifics by disulfide bridging.

Author information

1
Department of Chemistry, University College London , 20 Gordon Street, London, WC1H OAJ, United Kingdom.

Abstract

We report on a chemical platform to generate site-specific, homogeneous, antibody-antibody conjugates by targeting and bridging disulfide bonds. A bispecific antibody construct was produced in good yield through simple reduction and bridging of antibody fragment disulfide bonds, using a readily synthesized bis-dibromomaleimide cross-linker. Binding activity of antibodies was maintained, and in vitro binding of target antigens was observed. This technology is demonstrated through linking scFv and Fab antibody fragments, showing its potential for the construction of a diverse range of bispecifics.

PMID:
25033024
PMCID:
PMC4458859
DOI:
10.1021/bc5002467
[Indexed for MEDLINE]
Free PMC Article

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