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Int J Syst Evol Microbiol. 2014 Oct;64(Pt 10):3441-6. doi: 10.1099/ijs.0.063107-0. Epub 2014 Jul 16.

Deinococcus phoenicis sp. nov., an extreme ionizing-radiation-resistant bacterium isolated from the Phoenix Lander assembly facility.

Author information

1
Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California, Institute of Technology, Pasadena, CA 91109, USA vaishamp@jpl.nasa.gov.
2
Department of Biological Sciences, California State University Los Angeles, Los Angeles, CA 90032, USA.
3
Leibniz-Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, D-38124 Braunschweig, Germany.
4
German Aerospace Center, Institute of Aerospace Medicine, Cologne, Germany.
5
Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Chandigarh 160 036, India.
6
Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California, Institute of Technology, Pasadena, CA 91109, USA.

Abstract

A bacterial strain, designated 1P10ME(T), which was resistant to extreme doses of ionizing radiation, pale-pink, non-motile, and a tetrad-forming coccoid was isolated from a cleanroom at the Kennedy Space Center, where the Phoenix spacecraft was assembled. Strain 1P10ME(T) showed optimum growth at 30 °C, with a pH range for growth of 6.5-9.0 and was highly sensitive to sodium chloride, growing only in medium with no added NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1P10ME(T) represents a novel member of the genus Deinococcus, with low sequence similarities (<93.5%) to recognized species of the genus Deinococcus. The predominant cellular fatty acid was C15:1ω6c. This novel strain exhibits extreme resistance to gamma radiation (D10 >8 kGy) and UV (D10 >1000 Jm(-2)). The results of our polyphasic taxonomic analyses suggest that strain 1P10ME(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus phoenicis sp. nov. is proposed. The type strain is 1P10ME(T) ( = NRRL B-59546(T) = DSM 27173(T)).

PMID:
25030518
DOI:
10.1099/ijs.0.063107-0
[Indexed for MEDLINE]

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