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Dev Cell. 2014 Jul 14;30(1):86-94. doi: 10.1016/j.devcel.2014.06.006.

Cellular metabolism regulates contact sites between vacuoles and mitochondria.

Author information

1
Biochemistry Section, Department of Biology/Chemistry, University of Osnabrück, Barbarastrasse 13, 49076 Osnabrück, Germany.
2
Department of Cell Biology, Center for Molecular Medicine, University Medical Centre Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the Netherlands.
3
Institute for Biochemistry and Molecular Biology, ZBMZ, and BIOSS Centre for Biological Signalling Studies, University of Freiburg, Stefan-Meier-Strasse 17, 79104 Freiburg, Germany.
4
Molecular Cell Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
5
Biochemistry Section, Department of Biology/Chemistry, University of Osnabrück, Barbarastrasse 13, 49076 Osnabrück, Germany. Electronic address: cu@uos.de.

Abstract

Emerging evidence suggests that contact sites between different organelles form central hubs in the coordination of cellular physiology. Although recent work has emphasized the crucial role of the endoplasmic reticulum in interorganellar crosstalk, the cooperative behavior of other organelles is largely unexplored. Here, we identify a contact site named vCLAMP (vacuole and mitochondria patch) that integrates mitochondria with the lysosome-like vacuole and thus the endocytic pathway. vCLAMPs depend on the vacuolar HOPS tethering complex subunit Vps39/Vam6 and the Rab GTPase Ypt7, which also participate in membrane fusion at the vacuole. Intriguingly, vCLAMPs are located proximal to the ER-mitochondria encounter structure (ERMES) complexes, and an increase in vCLAMPs can rescue the growth defect of ERMES mutants. Importantly, the persistence of vCLAMPs is regulated by phosphorylation of Vps39 and is strongly reduced during respiratory growth. The identification of this organelle contact site reveals a physical and metabolic interconnection between the endocytic pathway and mitochondria.

PMID:
25026035
DOI:
10.1016/j.devcel.2014.06.006
[Indexed for MEDLINE]
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