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PeerJ. 2014 Jun 19;2:e427. doi: 10.7717/peerj.427. eCollection 2014.

Photobacterium sanctipauli sp. nov. isolated from bleached Madracis decactis (Scleractinia) in the St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil.

Author information

1
Institute of Biology, Federal University of Rio de Janeiro (UFRJ) , Rio de Janeiro , Brazil.
2
Botanical Garden Research Institute (JBRJ) , Rio de Janeiro , Brazil.
3
Department of Environment and Engineering, Federal University of Paraíba (UFPB) , Brazil.
4
BCCM/LMG Bacteria Collection, Ghent University , Ghent , Belgium ; Laboratory of Microbiology, Faculty of Sciences, Ghent University , Ghent , Belgium.
5
Institute of Biology, Federal University of Rio de Janeiro (UFRJ) , Rio de Janeiro , Brazil ; Laboratório de Sistemas Avançados de Gestão de Produção - SAGE - COPPE, Federal University of Rio de Janeiro , Rio de Janeiro , Brazil.

Abstract

Five novel strains of Photobacterium (A-394T, A-373, A-379, A-397 and A-398) were isolated from bleached coral Madracis decactis (scleractinian) in the remote St Peter & St Archipelago (SPSPA), Mid-Atlantic Ridge, Brazil. Healthy M. decactis specimens were also surveyed, but no strains were related to them. The novel isolates formed a distinct lineage based on the 16S rRNA, recA, and rpoA gene sequences analysis. Their closest phylogenetic neighbours were Photobacterium rosenbergii, P. gaetbulicola, and P. lutimaris, sharing 96.6 to 95.8% 16S rRNA gene sequence similarity. The novel species can be differentiated from the closest neighbours by several phenotypic and chemotaxonomic markers. It grows at pH 11, produces tryptophane deaminase, presents the fatty acid C18:0, but lacks C16:0 iso. The whole cell protein profile, based in MALDI-TOF MS, distinguished the strains of the novel species among each other and from the closest neighbors. In addition, we are releasing the whole genome sequence of the type strain. The name Photobacterium sanctipauli sp. nov. is proposed for this taxon. The G + C content of the type strain A-394(T) (= LMG27910(T) = CAIM1892(T)) is 48.2 mol%.

KEYWORDS:

Coral bleaching; Genomic taxonomy; New species; Photobacterium sanctipauli; St Paul’s rocks

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