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Microbiology. 2014 Oct;160(Pt 10):2295-303. doi: 10.1099/mic.0.080192-0. Epub 2014 Jul 14.

Lack of a surface layer in Tannerella forsythia mutants deficient in the type IX secretion system.

Author information

1
Division of Microbiology and Oral Infection, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 819-8588, Japan.
2
Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
3
Division of Microbiology and Oral Infection, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 819-8588, Japan knak@nagasaki-u.ac.jp.

Abstract

Tannerella forsythia, a Gram-negative anaerobic bacterium, is an important pathogen in periodontal disease. This bacterium possesses genes encoding all known components of the type IX secretion system (T9SS). T. forsythia mutants deficient in genes orthologous to the T9SS-encoding genes porK, porT and sov were constructed. All porK, porT and sov single mutants lacked the surface layer (S-layer) and expressed less-glycosylated versions of the S-layer glycoproteins TfsA and TfsB. In addition, these mutants exhibited decreased haemagglutination and increased biofilm formation. Comparison of the proteins secreted by the porK and WT strains revealed that the secretion of several proteins containing C-terminal domain (CTD)-like sequences is dependent on the porK gene. These results indicate that the T9SS is functional in T. forsythia and contributes to the translocation of CTD proteins to the cell surface or into the extracellular milieu.

PMID:
25023245
PMCID:
PMC4175972
DOI:
10.1099/mic.0.080192-0
[Indexed for MEDLINE]
Free PMC Article

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