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Curr Opin Chem Biol. 2014 Aug;21:89-95. doi: 10.1016/j.cbpa.2014.05.024. Epub 2014 Jul 11.

Photoclick chemistry: a fluorogenic light-triggered in vivo ligation reaction.

Author information

1
Department of Chemistry, State University of New York at Buffalo, Buffalo, NY 14260, USA.
2
Department of Chemistry, State University of New York at Buffalo, Buffalo, NY 14260, USA. Electronic address: qinglin@buffalo.edu.

Abstract

The ability to use chemical reactivity to monitor and control biomolecular processes with a spatial and temporal precision motivated the development of light-triggered in vivo chemistries. To this end, the photoinduced tetrazole-alkene cycloaddition, also termed 'photoclick chemistry' offers a very rapid chemical ligation platform for the manipulation of biomolecules and matrices in vivo. Here we outline the recent developments in the optimization of this chemistry, ranging from the search for substrates that offer two-photon photoactivatability, superior reaction kinetics, and/or genetic encodability, to the study of the reaction mechanism. The applications of the photoclick chemistry in protein labeling in vitro and in vivo as well as in preparing 'smart' hydrogels for 3D cell culture are highlighted.

PMID:
25022432
PMCID:
PMC4149939
DOI:
10.1016/j.cbpa.2014.05.024
[Indexed for MEDLINE]
Free PMC Article

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