Format

Send to

Choose Destination
Dev Cell. 2014 Jul 28;30(2):238-45. doi: 10.1016/j.devcel.2014.05.008. Epub 2014 Jul 10.

SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication.

Author information

1
Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
2
Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, NY 10065, USA.
3
Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan.
4
Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, NY 10065, USA. Electronic address: tsoum@mskcc.org.

Erratum in

  • Dev Cell. 2014 Aug 25;30(4):488.

Abstract

Centrioles are 9-fold symmetric structures duplicating once per cell cycle. Duplication involves self-oligomerization of the centriolar protein SAS-6, but how the 9-fold symmetry is invariantly established remains unclear. Here, we found that SAS-6 assembly can be shaped by preexisting (or mother) centrioles. During S phase, SAS-6 molecules are first recruited to the proximal lumen of the mother centriole, adopting a cartwheel-like organization through interactions with the luminal wall, rather than via their self-oligomerization activity. The removal or release of luminal SAS-6 requires Plk4 and the cartwheel protein STIL. Abolishing either the recruitment or the removal of luminal SAS-6 hinders SAS-6 (or centriole) assembly at the outside wall of mother centrioles. After duplication, the lumen of engaged mother centrioles becomes inaccessible to SAS-6, correlating with a block for reduplication. These results lead to a proposed model that centrioles may duplicate via a template-based process to preserve their geometry and copy number.

PMID:
25017693
PMCID:
PMC4116473
DOI:
10.1016/j.devcel.2014.05.008
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center