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Dev Cell. 2014 Jul 28;30(2):123-36. doi: 10.1016/j.devcel.2014.05.012. Epub 2014 Jul 10.

Spatial regionalization and heterochrony in the formation of adult pallial neural stem cells.

Author information

1
Institute of Neurobiology A. Fessard, Laboratory of Neurobiology and Development, CNRS UPR3294, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, 91198 Gif-sur-Yvette, France.
2
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 2213 Garland Ave, Nashville, TN 37232, USA.
3
Medical Research Council Centre for Developmental Neurobiology, King's College London, London SE1 1UL, UK.
4
Institute of Neurobiology A. Fessard, Laboratory of Neurobiology and Development, CNRS UPR3294, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, 91198 Gif-sur-Yvette, France. Electronic address: bally-cuif@inaf.cnrs-gif.fr.
5
Institute of Neurobiology A. Fessard, Laboratory of Neurobiology and Development, CNRS UPR3294, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, 91198 Gif-sur-Yvette, France. Electronic address: foucher@inaf.cnrs-gif.fr.

Abstract

Little is known on the embryonic origin and related heterogeneity of adult neural stem cells (aNSCs). We use conditional genetic tracing, activated in a global or mosaic fashion by cell type-specific promoters or focal laser uncaging, coupled with gene expression analyses and Notch invalidations, to address this issue in the zebrafish adult telencephalon. We report that the germinal zone of the adult pallium originates from two distinct subtypes of embryonic progenitors and integrates two modes of aNSC formation. Dorsomedial aNSCs derive from the amplification of actively neurogenic radial glia of the embryonic telencephalon. On the contrary, the lateral aNSC population is formed by stepwise addition at the pallial edge from a discrete neuroepithelial progenitor pool of the posterior telencephalic roof, activated at postembryonic stages and persisting lifelong. This dual origin of the pallial germinal zone allows the temporally organized building of pallial territories as a patchwork of juxtaposed compartments.

PMID:
25017692
DOI:
10.1016/j.devcel.2014.05.012
[Indexed for MEDLINE]
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