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Nucleic Acids Res. 1989 Jun 26;17(12):4731-44.

Molecular characterization of a gene encoding a photolyase from Streptomyces griseus.

Author information

1
Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan.

Abstract

By using a synthetic DNA probe derived from an amino acid sequence in the most conserved region of three known photolyases (Escherichia coli, Anacystis nidulans and Saccharomyces cerevisiae), we isolated a DNA fragment containing two long open reading frames (ORFs) from a genomic DNA library of Streptomyces griseus. One ORF encodes a polypeptide of 455 amino acids (Mr 50594), which exhibits substantial similarities with the other three photolyases. Photoreactivation-repair deficient E. coli cells could be converted into photoreactivatable ones by introduction of plasmids harboring this ORF, indicating that this is the photolyase gene of S. griseus. The deduced aa sequence of Streptomyces photolyase was most similar to that of E. coli. The putative DNA binding site as well as cofactor binding regions were proposed.

PMID:
2501760
PMCID:
PMC318028
DOI:
10.1093/nar/17.12.4731
[Indexed for MEDLINE]
Free PMC Article

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