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Toxicol In Vitro. 2014 Dec;28(8):1349-58. doi: 10.1016/j.tiv.2014.06.016. Epub 2014 Jul 9.

In vitro cytotoxicity of hydrothermally synthesized ZnO nanoparticles on human periodontal ligament fibroblast and mouse dermal fibroblast cells.

Author information

1
Ankara University Stem Cell Institute, Ankara 06100, Turkey; Ankara University Faculty of Science, Ankara 06100, Turkey.
2
Ankara University Faculty of Science, Ankara 06100, Turkey.
3
Ankara University Stem Cell Institute, Ankara 06100, Turkey; Ankara University Faculty of Science, Ankara 06100, Turkey. Electronic address: elcin@ankara.edu.tr.

Abstract

The use of metal oxide nanoparticles (NPs) in industrial applications has been expanding, as a consequence, risk of human exposure increases. In this study, the potential toxic effects of zinc oxide (ZnO) NPs on human periodontal ligament fibroblast cells (hPDLFs) and on mouse dermal fibroblast cells (mDFs) were evaluated in vitro. We synthesized ZnO NPs (particle size; 7-8 nm) by the hydrothermal method. Characterization assays were performed with atomic force microscopy, Braun-Emmet-Teller analysis, and dynamic light scattering. The hPDLFs and mDFs were incubated with the NPs with concentrations of 0.1, 1, 10, 50 and 100 μg/mL for 6, 24 and 48h. Under the control and NP-exposed conditions, we have made different types of measurements for cell viability and morphology, membrane leakage and intracellular reactive oxygen species generation. Also, we monitored cell responses to ZnO NPs using an impedance measurement system in real-time. While the morphological changes were visualized using scanning electron microscopy, the subcellular localization of NPs was investigated by transmission electron microscopy. Results indicated that ZnO NPs have significant toxic effects on both of the primary fibroblastic cells at concentrations of ∼50-100 μg/mL. The cytotoxicity of ZnO NPs on fibroblasts depended on concentration and duration of exposure.

KEYWORDS:

Dermal fibroblasts; Impedance measurement; Nanoparticles; Periodontal ligament fibroblasts; Toxicity; Zinc oxide

PMID:
25016134
DOI:
10.1016/j.tiv.2014.06.016
[Indexed for MEDLINE]

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