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J Microbiol Methods. 2014 Oct;105:88-91. doi: 10.1016/j.mimet.2014.07.004. Epub 2014 Jul 9.

Rapid, sensitive and specific detection of OXA-48-like-producing Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Author information

1
Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France; Centre de Ressources Biologiques - Ferdinand Cabanne, Centre Hospitalier Régional Universitaire, Besançon, France.
2
Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France.
3
Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France; Centre de Ressources Biologiques - Ferdinand Cabanne, Centre Hospitalier Régional Universitaire, Besançon, France. Electronic address: dhocquet@chu-besancon.fr.

Abstract

The emergence of Enterobacteriaceae producing the class D carbapenemase OXA-48 has become a public health concern, particularly in southern Europe and in the Mediterranean area. The existing MALDI-TOF MS assays that detect carbapenemase activity have disregarded the Enterobacteriaceae producing OXA-48-like enzymes. Here, we assessed the ability of an optimized MALDI-TOF MS imipenem hydrolysis assay to detect OXA-48-like-producing Enterobacteriaceae. We tested 372 non duplicate isolates of Enterobacteriaceae from 9 species, among which 92 harbored a blaOXA-48-like gene and 180 were non-susceptible to ertapenem. The vast majority (98.9%) of the OXA-48-like producers were detected and 97.8% of the ertapenem non-susceptible isolates were correctly classified. From the agar plate to the final result, the turnaround time of the test was 90min. This quick, easy and accurate MALDI-TOF MS test can expedite the implementation of hygiene measures to prevent dissemination of OXA-48-like-producing Enterobacteriaceae and limit outbreaks.

KEYWORDS:

Carbapenemase; Infection control; Klebsiella pneumoniae; MALDI-TOF MS

PMID:
25016131
DOI:
10.1016/j.mimet.2014.07.004
[Indexed for MEDLINE]

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