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Chem Commun (Camb). 2014 Aug 28;50(67):9591-4. doi: 10.1039/c4cc04491f.

Pyrazine-derived disulfide-reducing agent for chemical biology.

Author information

1
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA. rtraines@wisc.edu.

Abstract

For fifty years, dithiothreitol (DTT) has been the preferred reagent for the reduction of disulfide bonds in proteins and other biomolecules. Herein we report on the synthesis and characterization of 2,3-bis(mercaptomethyl)pyrazine (BMMP), a readily accessible disulfide-reducing agent with reactivity under biological conditions that is markedly superior to DTT and other known reagents.

PMID:
25014913
PMCID:
PMC4237594
DOI:
10.1039/c4cc04491f
[Indexed for MEDLINE]
Free PMC Article

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