Abstract
Herein, we describe a fast and robust method for achieving (68)Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels-Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.
MeSH terms
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Animals
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Antibodies, Monoclonal, Humanized / chemistry*
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Antibodies, Monoclonal, Humanized / immunology
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Cell Line, Tumor
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Cell Transformation, Neoplastic
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Cetuximab
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ErbB Receptors / immunology
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Gallium Radioisotopes
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Humans
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Isotope Labeling / methods*
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Mice
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Positron-Emission Tomography*
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Time Factors
Substances
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Antibodies, Monoclonal, Humanized
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Gallium Radioisotopes
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ErbB Receptors
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Cetuximab