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Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2014;31(10):1678-87. doi: 10.1080/19440049.2014.943304. Epub 2014 Aug 30.

Probiotic lactic acid bacteria detoxify N-nitrosodimethylamine.

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a Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences , Lodz University of Technology , Lodz , Poland.


Humans can be exposed to N-nitroso compounds (NOCs) due to many environmental sources, as well as endogenous formation. The main nitrosamine found in food products and also synthesised in vivo by intestinal microbiota is N-nitrosodimethylamine (NDMA). It can cause cancer of the stomach, kidney and colon. The effect of four probiotic Lactobacillus strains on NDMA was studied under different culture conditions (24 h in MRS, 168 h in modified MRS N, and 168 h in phosphate buffer). HPLC and GC-TEA methods were used for NDMA determination in supernatants. The influence of lactic acid bacteria on NDMA genotoxicity was investigated by means of the comet assay. Additionally, the effect of NDMA (2-100 µg ml⁻¹) on the growth and survival of the probiotic strains was studied. The results indicate that the bacteria decreased NDMA concentration by up to 50%, depending on the culture conditions, time of incubation, NDMA concentration, pH and bacterial strain. Lb. brevis 0945 lowered the concentration and genotoxicity of NDMA most effectively by up to 50%. This could be due to either adsorption or metabolism. The growth and survival of the bacteria was not affected by any of the tested NDMA concentrations.


Lactobacillus; N-nitrosodimethylamine; detoxification; probiotics

[Indexed for MEDLINE]

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