Format

Send to

Choose Destination
Virology. 2014 Jul;460-461:55-65. doi: 10.1016/j.virol.2014.04.032. Epub 2014 May 29.

Sequestration of human cytomegalovirus by human renal and mammary epithelial cells.

Author information

1
Institute for Medical Immunology, Université Libre de Bruxelles, Rue A. Bolland 8, B-6041 Charleroi, Belgium.
2
Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, Belgium.
3
ImmuneHealth, Rue A. Bolland 8, B-6041 Charleroi, Belgium.
4
Department of Obstetrics and Gynecology, Erasme Hospital, Route de Lennik 808, 1070 Brussels, Belgium.
5
Laboratory of Molecular Parasitology, Institut de Biologie et Médecine Moléculaires, Université Libre de Bruxelles, Gosselies, Belgium.
6
Pathology Department, U.Z. Leuven, Minderbroedersstraat 12, Leuven, Belgium.
7
Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, Belgium. Electronic address: Robert.Snoeck@Rega.kuleuven.be.
8
Institute for Medical Immunology, Université Libre de Bruxelles, Rue A. Bolland 8, B-6041 Charleroi, Belgium; ImmuneHealth, Rue A. Bolland 8, B-6041 Charleroi, Belgium. Electronic address: arnaud.marchant@ulb.ac.be.

Abstract

Urine and breast milk represent the main routes of human cytomegalovirus (HCMV) transmission but the contribution of renal and mammary epithelial cells to viral excretion remains unclear. We observed that kidney and mammary epithelial cells were permissive to HCMV infection and expressed immediate early, early and late antigens within 72 h of infection. During the first 24 h after infection, high titers of infectious virus were measured associated to the cells and in culture supernatants, independently of de novo synthesis of virus progeny. This phenomenon was not observed in HCMV-infected fibroblasts and suggested the sequestration and the release of HCMV by epithelial cells. This hypothesis was supported by confocal and electron microscopy analyses. The sequestration and progressive release of HCMV by kidney and mammary epithelial cells may play an important role in the excretion of the virus in urine and breast milk and may thereby contribute to HCMV transmission.

KEYWORDS:

Antibody; HCMV glycoprotein B; Human cytomegalovirus; Interferon-gamma; Mammary epithelial cell; Renal epithelial cell; Viral excretion

PMID:
25010270
DOI:
10.1016/j.virol.2014.04.032
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center