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J Proteomics. 2014 Dec 5;111:16-29. doi: 10.1016/j.jprot.2014.06.029. Epub 2014 Jul 5.

Quantitative proteomic analysis of Huh-7 cells infected with Dengue virus by label-free LC-MS.

Author information

1
Instituto Nacional de Salud Pública, Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Universidad No. 655 Colonia Santa María Ahuacatitlán, Cerrada Los Pinos y Caminera, C.P. 62100, Cuernavaca, Mor., México.
2
Department of Pharmaceutical Chemistry, University of California at San Francisco, 600 16th Street, 94158-2517, San Francisco, CA, USA.
3
Laboratorio Universitario de Proteómica (LUP), Instituto de Biotecnología-UNAM, Av. Universidad, 2001, Col Chamilpa, C.P. 62210, Cuernavaca, Mor., México.
4
Laboratorio Universitario de Proteómica (LUP), Instituto de Biotecnología-UNAM, Av. Universidad, 2001, Col Chamilpa, C.P. 62210, Cuernavaca, Mor., México. Electronic address: fbatista@ibt.unam.mx.

Abstract

Dengue is an important and growing public health problem worldwide with an estimated 100million new clinical cases annually. Currently, no licensed drug or vaccine is available. During natural infection in humans, liver cells constitute one of the main targets of dengue virus (DENV) replication. However, a clear understanding of dengue pathogenesis remains elusive. In order to gain a better reading of the cross talk between virus and host cell proteins, we used a proteomics approach to analyze the host response to DENV infection in a hepatic cell line Huh-7. Differences in proteome expression were assayed 24h post-infection using label-free LC-MS. Quantitative analysis revealed 155 differentially expressed proteins, 64 of which were up-regulated and 91 down-regulated. These results reveal an important decrease in the expression of enzymes involved in the glycolytic pathway, citrate cycle, and pyruvate metabolism. This study provides large-scale quantitative information regarding protein expression in the early stages of infection that should be useful for better compression of the pathogenesis of dengue.

BIOLOGICAL SIGNIFICANCE:

Dengue infection involves alterations in the homeostasis of the host cell. Defining the interactions between virus and cell proteins should provide a better understanding of how viruses propagate and cause disease. Here, we present for the first time the proteomic analysis of hepatocytes (Huh-7 cells) infected with DENV-2 by label-free LC-MS.

KEYWORDS:

Dengue infection; Huh-7 cells; Label-free LC–MS

PMID:
25009145
DOI:
10.1016/j.jprot.2014.06.029
[Indexed for MEDLINE]
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