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J Virol. 2014 Sep;88(18):10958-62. doi: 10.1128/JVI.00870-14. Epub 2014 Jul 9.

Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography.

Author information

1
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
2
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
3
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA ss1@nih.gov.

Abstract

The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.

PMID:
25008940
PMCID:
PMC4178867
DOI:
10.1128/JVI.00870-14
[Indexed for MEDLINE]
Free PMC Article

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