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J Biol Chem. 2014 Aug 22;289(34):23701-11. doi: 10.1074/jbc.M114.576272. Epub 2014 Jul 9.

A WXW motif is required for the anticancer activity of the TAT-RasGAP317-326 peptide.

Author information

1
From the Department of Physiology, University of Lausanne, 1005 Lausanne, Switzerland.
2
the Molecular Modeling Group, Swiss Institute of Bioinformatics (SIB), Quartier Sorge, Bâtiment Génopode, 1015 Lausanne, Switzerland.
3
the Department of Fundamental Microbiology, University of Lausanne, 1015 Lausanne, Switzerland, and.
4
the Institute of Cell Biology and Immunology, University of Stuttgart, 70569 Stuttgart, Germany.
5
From the Department of Physiology, University of Lausanne, 1005 Lausanne, Switzerland, Christian.Widmann@unil.ch.

Abstract

TAT-RasGAP317-326, a cell-permeable 10-amino acid-long peptide derived from the N2 fragment of p120 Ras GTPase-activating protein (RasGAP), sensitizes tumor cells to apoptosis induced by various anticancer therapies. This RasGAP-derived peptide, by targeting the deleted in liver cancer-1 (DLC1) tumor suppressor, also hampers cell migration and invasion by promoting cell adherence and by inhibiting cell movement. Here, we systematically investigated the role of each amino acid within the RasGAP317-326 sequence for the anticancer activities of TAT-RasGAP317-326. We report here that the first three amino acids of this sequence, tryptophan, methionine, and tryptophan (WMW), are necessary and sufficient to sensitize cancer cells to cisplatin-induced apoptosis and to reduce cell migration. The WMW motif was found to be critical for the binding of fragment N2 to DLC1. These results define the interaction mode between the active anticancer sequence of RasGAP and DLC1. This knowledge will facilitate the design of small molecules bearing the tumor-sensitizing and antimetastatic activities of TAT-RasGAP317-326.

KEYWORDS:

Anticancer Peptide; Cell Migration; Cell-penetrating peptide (CPP); Deleted in Liver Cancer 1 (DLC1); Docking; GTPase-activating Protein (GAP); Peptides; RasGAP; Sensitizer; TAT-RasGAP317–326

PMID:
25008324
PMCID:
PMC4156047
DOI:
10.1074/jbc.M114.576272
[Indexed for MEDLINE]
Free PMC Article

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