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Am J Chin Med. 2014;42(4):935-47. doi: 10.1142/S0192415X14500591.

Suppressive effects of britanin, a sesquiterpene compound isolated from Inulae flos, on mast cell-mediated inflammatory responses.

Author information

1
Research and Development Division, Korean Promotion Institute for Traditional Medicine Industry, Gyeongsan 710-210, Republic of Korea.

Abstract

Mast cells are central players in immediate-type hypersensitvity and inflammatory responses. In the present study, the effects of britanin on the passive cutaneous anaphylaxis (PCA) reaction in mice and on the phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced production of pro-inflammatory cytokines in human mast cell line (HMC-1) were evaluated. The oral administration of britanin (10-20 mg/kg) decreased the mast cell-mediated PCA reaction in IgE-sensitized mice. In the activity and mechanism of britanin in vitro assay, britanin suppressed the gene expression and secretion of pro-inflammatory cytokines in a dose-dependent manner in HMC-1. In addition, britanin attenuated PMACI-induced activation of NF-κB as indicated by the inhibition of the degradation of IκBα, nuclear translocation of NF-κB, NF-κB/DNA binding activity assay, and blocked the phosphorylation of p38 MAP kinase, in a dose-dependent manner. We conclude that britanin may have potential as a treatment for allergic-inflammatory diseases.

KEYWORDS:

Britanin; Mast Cells; Mitogen-Activated Protein (MAP) Kinases; Nuclear Factor (NF)-κB; Passive Cutaneous Anaphylaxis (PCA); Pro-Inflammatory Cytokines

PMID:
25004884
DOI:
10.1142/S0192415X14500591
[Indexed for MEDLINE]

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