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Am J Chin Med. 2014;42(4):891-904. doi: 10.1142/S0192415X14500566.

Anti-inflammatory effect of Lycii radicis in LPS-stimulated RAW 264.7 macrophages.

Author information

1
Department of Rehabilitation Medicine, Dongguk University, Gyeongju 780-350, Republic of Korea.

Abstract

The root bark of Lycium barbarum (Lycii radicis cortex, LRC) is used as a cooling agent for fever and night sweats in East Asian traditional medicine. The inhibitory effect of LRC water extract on inflammation is unknown. In this study, the anti-inflammatory effect of LRC was investigated in lipopolysaccharide (LPS)-stimulated mouse macrophage, RAW 264.7 cells. LRC extract significantly decreased the LPS-induced production of inflammatory mediators, nitric oxide (NO), prostaglandin (PG) E2 and pro-inflammatory cytokines, interleukin (IL)-1β and IL-6 in the cells. In addition, LRC extract inhibited the LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 mRNA and protein, and inflammatory cytokines mRNA in the cells. The action mechanism of LRC underlies the blocking of LPS-mediated p38 and Jun N-terminal kinase (JNK), mitogen-activated protein kinases (MAPKs), and the nuclear factor (NF)-κB signaling pathway. These results indicate that LRC extract inhibits the inflammatory response in activated macrophages by down-regulating the transcription levels of inflammatory mediators and blocking the MAPKs and NF-κB pathway.

KEYWORDS:

Inflammation; Lycii radicis Cortex; Mitogen-Activated Protein Kinases; Nuclear Factor κB; RAW 264.7 Macrophages

PMID:
25004881
DOI:
10.1142/S0192415X14500566
[Indexed for MEDLINE]

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