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Mediators Inflamm. 2014;2014:987017. doi: 10.1155/2014/987017. Epub 2014 Jun 9.

Treadmill training increases SIRT-1 and PGC-1 α protein levels and AMPK phosphorylation in quadriceps of middle-aged rats in an intensity-dependent manner.

Author information

1
Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
2
Faculty of Applied Sciences, University of Campinas (UNICAMP), 13083-872 Limeira, SP, Brazil ; Post Graduation Program in Motricity Science, Instituto de Biociências, São Paulo State University (UNESP), 13506-900 Rio Claro, SP, Brazil.
3
Post Graduation Program in Motricity Science, Instituto de Biociências, São Paulo State University (UNESP), 13506-900 Rio Claro, SP, Brazil.
4
Human Movement Laboratory, São Judas Tadeu University, 03166-000 São Paulo, SP, Brazil.
5
Immunometabolism Research Group, Department of Physical Education, São Paulo State University (UNESP), 19060-900 Presidente Prudente, SP, Brazil.
6
Faculty of Applied Sciences, University of Campinas (UNICAMP), 13083-872 Limeira, SP, Brazil.
7
School of Physical Education and Sport of Ribeirão Preto, São Paulo University (USP), 14040-907 Ribeirão Preto, SP, Brazil.

Abstract

The present study investigated the effects of running at 0.8 or 1.2 km/h on inflammatory proteins (i.e., protein levels of TNF- α , IL-1 β , and NF- κ B) and metabolic proteins (i.e., protein levels of SIRT-1 and PGC-1 α , and AMPK phosphorylation) in quadriceps of rats. Male Wistar rats at 3 (young) and 18 months (middle-aged rats) of age were divided into nonexercised (NE) and exercised at 0.8 or 1.2 km/h. The rats were trained on treadmill, 50 min per day, 5 days per week, during 8 weeks. Forty-eight hours after the last training session, muscles were removed, homogenized, and analyzed using biochemical and western blot techniques. Our results showed that: (a) running at 0.8 km/h decreased the inflammatory proteins and increased the metabolic proteins compared with NE rats; (b) these responses were lower for the inflammatory proteins and higher for the metabolic proteins in young rats compared with middle-aged rats; (c) running at 1.2 km/h decreased the inflammatory proteins and increased the metabolic proteins compared with 0.8 km/h; (d) these responses were similar between young and middle-aged rats when trained at 1.2 km. In summary, the age-related increases in inflammatory proteins, and the age-related declines in metabolic proteins can be reversed and largely improved by treadmill training.

PMID:
25002755
PMCID:
PMC4070581
DOI:
10.1155/2014/987017
[Indexed for MEDLINE]
Free PMC Article

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