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Anal Chim Acta. 2014 Jul 21;837:1-15. doi: 10.1016/j.aca.2014.04.055. Epub 2014 May 2.

Aptamer binding assays for proteins: the thrombin example--a review.

Author information

1
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, T6G 2G3, Canada.
2
Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada.
3
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, T6G 2G3, Canada; Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada. Electronic address: xc.le@ualberta.ca.

Abstract

Experimentally selected single-stranded DNA and RNA aptamers are able to bind to specific target molecules with high affinity and specificity. Many analytical methods make use of affinity binding between the specific targets and their aptamers. In the development of these methods, thrombin is the most frequently used target molecule to demonstrate the proof-of-principle. This paper critically reviews more than one hundred assays that are based on aptamer binding to thrombin. This review focuses on homogeneous binding assays, electrochemical aptasensors, and affinity separation techniques. The emphasis of this review is placed on understanding the principles and unique features of the assays. The principles of most assays for thrombin are applicable to the determination of other molecular targets.

KEYWORDS:

Affinity separation; Aptamers; Binding-induced DNA assembly; Catalytic amplification; Homogeneous binding assays; Thrombin

PMID:
25000852
DOI:
10.1016/j.aca.2014.04.055
[Indexed for MEDLINE]
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