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Exp Parasitol. 2014 Nov;145 Suppl:S73-7. doi: 10.1016/j.exppara.2014.06.021. Epub 2014 Jul 3.

Monitoring of in vitro dynamics of Acanthamoeba strains isolated from infected eyes as a useful tool in keratitis management.

Author information

1
Department of Medical Biology, Medical University of Warsaw, 73 Nowogrodzka Str., 02-018 Warsaw, Poland. Electronic address: lidia.chomicz@wum.edu.pl.
2
Department of Medical Biology, Medical University of Warsaw, 73 Nowogrodzka Str., 02-018 Warsaw, Poland.
3
Department of Ophthalmology, SPKSO Ophthalmic Hospital, Medical University of Warsaw, 13 Sierakowskiego Str., 03-709 Warsaw, Poland.
4
Department of Tropical and Parasitic Diseases, Medical University of Gdansk, 9b Powstania Styczniowego Str., 81-519 Gdynia, Poland.

Abstract

Free-living amoebae of Acanthamoeba genus are ubiquitous in various parts of the world. Some species of these amoebozoans present a serious risk to human health as the causative agents of vision-threatening diseases, Acanthamoeba keratitis. Correct diagnosis requires both a clinical examination of the cornea and amoebic form identification in affected eyes. Despite advances in pharmacotherapy, the infection is difficult to diagnose and to threat. Population dynamics of five different Acanthamoeba strains cultured in vitro under bacteria-free condition in BSC medium, was monitored in terms of diagnostic and therapeutic management. The range of protozoan number in the exponential growth phase, the morpho-physiological status of amoeba forms and their ability to multiply were evaluated. Results of the studies revealed that early and continued monitoring of the strains maintained in an axenic culture showed correlation between the dynamics of cultivated amoebae and the course of the disease, differences in response to pharmacotherapy and the surgical management efficacy. Concluding, the in vitro monitoring of dynamics of Acanthamoeba strains isolated from infected corneas may be important not only for proper diagnosis but also as a useful tool in keratitis management and therapeutic prognosis.

KEYWORDS:

In vitro dynamics; Keratitis; Pathogenic Acanthamoeba strains

PMID:
24999093
DOI:
10.1016/j.exppara.2014.06.021
[Indexed for MEDLINE]
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