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Bioelectrochemistry. 2014 Oct;99:53-6. doi: 10.1016/j.bioelechem.2014.06.008. Epub 2014 Jun 24.

A simple method for determination of D-penicillamine on the carbon paste electrode using cupric ions.

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Faculty of Chemistry, Iran University of Science and Technology, Tehran 1684613114, Iran. Electronic address:
Department of Chemistry, Islamic Azad University, Firoozabad Branch, P.O. Box 74715-117, Firoozabad, Fars, Iran.
Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, Hamedan 65174, Iran.
Faculty of Chemistry, Iran University of Science and Technology, Tehran 1684613114, Iran.


The interaction of D-penicillamine (PA) with copper at the carbon paste electrode (CPE) in the presence of cupric ions (Cu(2+)) was used for the determination of PA at very low potential (0.1 V vs. Ag/AgCl) without applying of any modifier. The electrochemical response of copper is changed considerably in the presence of negligible amount of PA. In this report some important parameters, such as pH effect, Cu(2+) concentration and scan rate are studied, which the selected conditions were acetate buffer (pH=6) and 1 mM Cu(2+). The linear range for PA was from 1.0×10(-6) to 1.0×10(-4) M with an experimental detection limit of 1.0×10(-7) M. The relative standard deviation for 6 measurements was 3.8%. The interfering effects of some important inorganic ions were investigated, which there was no significant effect on the PA measurements. Also three organic interferences including ascorbic acid (AA), uric acid (UA) and l-cysteine (Cys) were examined, which the effect of AA was not notable, the interference of UA was moderate and for Cys was significant, but moderate at the levels which fined in the urine samples. This method was applied successfully for the determination of PA in urine sample.


Carbon paste electrode; Cupric ion; Urine sample; d-penicillamine

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