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Dev Cell. 2014 Jul 14;30(1):23-35. doi: 10.1016/j.devcel.2014.05.023. Epub 2014 Jul 3.

Uniformity of nucleosome preservation pattern in Mammalian sperm and its connection to repetitive DNA elements.

Author information

1
Research Centre for Biosystems, Land Use and Nutrition, IFZ, Justus Liebig University Giessen, 35392 Giessen, Germany.
2
Clinic and Polyclinic of Urology, Pediatric Urology and Andrology, Department of Molecular Andrology, Justus Liebig University Giessen, 35392 Giessen, Germany.
3
Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-University Munich, 81377 Munich, Germany.
4
Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-University Munich, 81377 Munich, Germany.
5
Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-University Munich, 81377 Munich, Germany; Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-University Munich, 81377 Munich, Germany.
6
Clinic and Polyclinic of Urology, Pediatric Urology and Andrology, Department of Molecular Andrology, Justus Liebig University Giessen, 35392 Giessen, Germany. Electronic address: undraga.schagdarsurengin@chiru.med.uni-giessen.de.

Abstract

Nucleosome-to-protamine exchange during mammalian spermiogenesis is essential for compaction and protection of paternal DNA. It is interesting that, depending on the species, 1% to 15% of nucleosomes are retained, but the generalizability and biological function of this retention are unknown. Here, we show concordantly in human and bovine that nucleosomes remained in sperm chromatin predominantly within distal intergenic regions and introns and associated with centromere repeats and retrotransposons (LINE1 and SINEs). In contrast, nucleosome depletion concerned particularly exons, 5'-UTR, 3'-UTR, TSS, and TTS and was associated with simple and low-complexity repeats. Overlap of human and bovine genes exhibiting nucleosome preservation in the promoter and gene body revealed a significant enrichment of signal transduction and RNA- and protein-processing factors. Our study demonstrates the genome-wide uniformity of the nucleosome preservation pattern in mammalian sperm and its connection to repetitive DNA elements and suggests a function in preimplantation processes for paternally derived nucleosomes.

PMID:
24998597
DOI:
10.1016/j.devcel.2014.05.023
[Indexed for MEDLINE]
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