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Genetics. 2014 Sep;198(1):383-96. doi: 10.1534/genetics.114.165613. Epub 2014 Jul 3.

Mapping-by-sequencing identifies HvPHYTOCHROME C as a candidate gene for the early maturity 5 locus modulating the circadian clock and photoperiodic flowering in barley.

Author information

1
Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany.
2
Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Seeland (OT Gatersleben), Germany.
3
Department of Crystallography, A. Mickiewicz University, 60-780 Poznan, Poland.
4
Department of Crystallography, A. Mickiewicz University, 60-780 Poznan, Poland Department of Biology, University of York, YO10 5DD York, United Kingdom.
5
Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany Institute of Plant Genetics, Heinrich Heine University, 40225 Düsseldorf, Germany Cluster of Excellence on Plant Sciences "From Complex Traits Towards Synthetic Modules" 40225 Düsseldorf, Germany korff@mpipz.mpg.de.

Abstract

Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under noninductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.

KEYWORDS:

barley; circadian clock; flowering; photoperiod; phytochrome

PMID:
24996910
PMCID:
PMC4174949
DOI:
10.1534/genetics.114.165613
[Indexed for MEDLINE]
Free PMC Article

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