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FEBS Lett. 2014 Aug 25;588(17):2881-9. doi: 10.1016/j.febslet.2014.06.056. Epub 2014 Jul 1.

Gatekeeper tyrosine phosphorylation is autoinhibitory for Symbiosis Receptor Kinase.

Author information

1
Department of Biochemistry, University of Calcutta, Kolkata, India.
2
Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700 032, India.
3
Department of Biochemistry, University of Calcutta, Kolkata, India. Electronic address: maitrayee_d@hotmail.com.

Abstract

Plant receptor-like kinases (RLKs) are distinguished by having a tyrosine in the 'gatekeeper' position. Previously we reported Symbiosis Receptor Kinase from Arachis hypogaea (AhSYMRK) to autophosphorylate on the gatekeeper tyrosine (Y670), though this phosphorylation was not necessary for the kinase activity. Here we report that recombinant catalytic domain of AhSYMRK with a phosphomimic substitution in the gatekeeper position (Y670E) is catalytically almost inactive and is conformationally quite distinct from the corresponding native enzyme. Additionally, we show that gatekeeper-phosphorylated AhSYMRK polypeptides are inactive and depletion of this inactive form leads to activation of intramolecular autophosphorylation of AhSYMRK. Together, our results suggest gatekeeper tyrosine autophosphorylation to be autoinhibitory for AhSYMRK.

KEYWORDS:

Autoinhibition; Autophosphorylation; Gatekeeper tyrosine; Symbiosis Receptor Kinase

PMID:
24996184
DOI:
10.1016/j.febslet.2014.06.056
[Indexed for MEDLINE]
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