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Exp Hematol. 2014 Aug;42(8):598-608. doi: 10.1016/j.exphem.2014.05.003. Epub 2014 Jul 1.

Cellular barcoding: a technical appraisal.

Author information

1
Molecular Medicine Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia. Electronic address: shalinhnaik@gmail.com.
2
Division of Immunology, Netherlands Cancer Institute, Amsterdam, The Netherlands. Electronic address: t.schumacher@nki.nl.
3
Division of Immunology, Netherlands Cancer Institute, Amsterdam, The Netherlands; Theoretical Biology and Bioinformatics, Utrecht University, Utrecht, The Netherlands. Electronic address: l.perie@nki.nl.

Abstract

Cellular barcoding involves the tagging of individual cells of interest with unique genetic heritable identifiers or barcodes and is emerging as a powerful tool to address individual cell fates on a large scale. However, as with many new technologies, diverse technical and analytical challenges have emerged. Here, we review those challenges and highlight both the power and limitations of cellular barcoding. We then illustrate the contribution of cellular barcoding to the understanding of hematopoiesis and outline the future potential of this technology.

PMID:
24996012
DOI:
10.1016/j.exphem.2014.05.003
[Indexed for MEDLINE]

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