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Head Neck. 2016 Jan;38(1):9-14. doi: 10.1002/hed.23842. Epub 2015 Jun 26.

Serum biomarkers for detection of head and neck squamous cell carcinoma.

Author information

1
Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
2
Division of Biostatistics, Johns Hopkins University School of Medicine, Baltimore, Maryland.
3
Department of Oncology, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
4
Milton J. Dance Head and Neck Center, Greater Baltimore Medical Center, Baltimore, Maryland.

Abstract

BACKGROUND:

Detection of hypermethylated circulating tumor DNA has the potential to be a minimally invasive, low cost, and reproducible method for cancer detection.

METHODS:

We evaluated serum from 100 patients with known head and neck squamous cell carcinoma (HNSCC) and 50 healthy control patients for 3 previously described methylation targets, endothelin receptor type B (EDNRB), cyclin-dependent kinase inhibitor 2A (CDKN2A or p16), and deleted in colorectal carcinoma (DCC), using quantitative methylation specific polymerase chain reaction (qMSPCR).

RESULTS:

EDNRB hypermethylation was identified in the serum of 10% of the patients with HNSCC but in none of the control patients. DCC hypermethylation was detected in 2 serum samples from patients with cancer that also amplified EDNRB and one of these samples also had p16 hypermethylation. EDNRB hypermethylation was statistically significant by Fisher's exact test (p = .03) when comparing HNSCC to controls.

CONCLUSIONS:

Serum EDNRB hypermethylation is a highly specific but not sensitive serum biomarker for HNSCC.

KEYWORDS:

epigenetics; head and neck cancer; head and neck squamous cell carcinoma (HNSCC); hypermethylation; screening

PMID:
24995714
PMCID:
PMC4317379
DOI:
10.1002/hed.23842
[Indexed for MEDLINE]
Free PMC Article

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