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Neurosci Lett. 2014 Aug 22;578:176-81. doi: 10.1016/j.neulet.2014.06.049. Epub 2014 Jun 30.

Three puncture sites used for in utero electroporation show no significantly different negative impacts during gene transfer into the embryonic mouse brain.

Author information

1
Bayi Brain Hospital, Bayi Clinical Medical Institute of the Southern Medical University, Beijing 100700, China; Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China.
2
Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China; Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
3
Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China.
4
Bayi Brain Hospital, Bayi Clinical Medical Institute of the Southern Medical University, Beijing 100700, China; Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China. Electronic address: zjxuruxiang@163.com.

Abstract

Although various ways to manipulate genes in vivo exist, in utero electroporation is a widely used technique, especially in the field of neural development due to its many advantages. In this study, we focused on direct comparison between three puncture sites during in utero electroporation on the death rate of embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis. We found no statistical significant differences between the three puncture methods in the death rate of embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis.

KEYWORDS:

Differentiation; In utero electroporation; Proliferation; Puncture site; Thickness of cortex

PMID:
24993303
DOI:
10.1016/j.neulet.2014.06.049
[Indexed for MEDLINE]

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