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Neuron. 2014 Jul 2;83(1):51-68. doi: 10.1016/j.neuron.2014.05.013.

CORTECON: a temporal transcriptome analysis of in vitro human cerebral cortex development from human embryonic stem cells.

Author information

1
Neural Stem Cell Institute, Rensselaer, NY 12144, USA.
2
Neural Stem Cell Institute, Rensselaer, NY 12144, USA; Department of Computer Science, College of Computing and Information, State University of New York, Albany, NY 12144, USA.
3
Allen Institute for Brain Science, Seattle, WA 98103, USA.
4
Neural Stem Cell Institute, Rensselaer, NY 12144, USA. Electronic address: sallytemple@neuralsci.org.
5
Neural Stem Cell Institute, Rensselaer, NY 12144, USA. Electronic address: chrisfasano@neuralsci.org.

Abstract

Many neurological and psychiatric disorders affect the cerebral cortex, and a clearer understanding of the molecular processes underlying human corticogenesis will provide greater insight into such pathologies. To date, knowledge of gene expression changes accompanying corticogenesis is largely based on murine data. Here we present a searchable, comprehensive, temporal gene expression data set encompassing cerebral cortical development from human embryonic stem cells (hESCs). Using a modified differentiation protocol that yields neurons suggestive of prefrontal cortex, we identified sets of genes and long noncoding RNAs that significantly change during corticogenesis and those enriched for disease-associations. Numerous alternatively spliced genes with varying temporal patterns of expression are revealed, including TGIF1, involved in holoprosencephaly, and MARK1, involved in autism. We have created a database (http://cortecon.neuralsci.org/) that provides online, query-based access to changes in RNA expression and alternatively spliced transcripts during human cortical development.

PMID:
24991954
DOI:
10.1016/j.neuron.2014.05.013
[Indexed for MEDLINE]
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