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J Neurosci. 2014 Jul 2;34(27):9088-95. doi: 10.1523/JNEUROSCI.0303-14.2014.

Loss of hnRNP K impairs synaptic plasticity in hippocampal neurons.

Author information

1
CNR Institute of Neuroscience, Department of Medical Biotechnology and Translational Medicine (BIOMETRA), University of Milan, Milan 20129, Italy.
2
CNR Institute of Neuroscience, Department of Medical Biotechnology and Translational Medicine (BIOMETRA), University of Milan, Milan 20129, Italy, Department of Brain and Behavioral Sciences, Neurophysiology Unit, University of Pavia, Pavia 27100, Italy, Brain Connectivity Center, C. Mondino National Neurological Institute, Pavia 27100, Italy, and.
3
IRCCS Istituto Auxologico Italiano, Department of Neurology and Laboratory of Neuroscience, Milan 20095, Italy.
4
Department of Brain and Behavioral Sciences, Neurophysiology Unit, University of Pavia, Pavia 27100, Italy.
5
Department of Brain and Behavioral Sciences, Neurophysiology Unit, University of Pavia, Pavia 27100, Italy, Brain Connectivity Center, C. Mondino National Neurological Institute, Pavia 27100, Italy, and.
6
CNR Institute of Neuroscience, Department of Medical Biotechnology and Translational Medicine (BIOMETRA), University of Milan, Milan 20129, Italy, s.bassani@in.cnr.it m.passafaro@in.cnr.it.

Abstract

Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is an RNA-binding protein implicated in RNA metabolism. Here, we investigated the role of hnRNP K in synapse function. We demonstrated that hnRNP K regulates dendritic spine density and long-term potentiation (LTP) in cultured hippocampal neurons from embryonic rats. LTP requires the extracellular signal-regulated kinase (ERK)1/2-mediated phosphorylation and cytoplasmic accumulation of hnRNP K. Moreover, hnRNP K knockdown prevents ERK cascade activation and GluA1-S845 phosphorylation and surface delivery, which are essential steps for LTP. These findings establish hnRNP K as a new critical regulator of synaptic transmission and plasticity in hippocampal neurons.

KEYWORDS:

LTP; dendritic spines; hnRNP K; neurons; synapse

PMID:
24990929
PMCID:
PMC6608249
DOI:
10.1523/JNEUROSCI.0303-14.2014
[Indexed for MEDLINE]
Free PMC Article

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