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Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Jun;22(3):717-22. doi: 10.7534/j.issn.1009-2137.2014.03.027.

[Effect of 5-aza-2'-deoxycytidine on DAPK gene expression in human HL-60 cells].

[Article in Chinese]

Author information

1
Department of Pediatrics, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
2
Department of Pediatrics, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China. E-mail: lwjlyfy@sina.com.

Abstract

This study was aimed to investigate the effect of methylation transferase inhibitor 5-aza-2'-deoxycytidine (5-aza-2dC) of different concentrations on the apoptosis of human acute myeloid leukemia (AML) cell line HL-60 and the expression of DAPK gene in HL-60 cells, as well as to explore the possible anti-AML mechanism of 5-aza-2dC. HL-60 cells were treated by 5-aza-2dC of different concentrations. The effect of 5-aza-2dC on the HL-60 cell morphology was observed by Wright's staining. The effect of 5-aza-2dC on HL-60 cell apoptosis and DAPK mRNA expression was detected by flow cytometry and reverse transcription-polymerize chain reaction (RT-PCR) respectively. The results showed that the 5-aza-2dC induced the apoptosis of HL-60 cells in a concentration-dependent manner; the 5-aza-2dC increased the expression levels of DAPK mRNA in HL-60 cells in a concentration-dependent manner. It is concluded that the apoptosis rate of HL-60 cells and DAPK mRNA expression level displayed a rising trend with 5-aza-2dC concentration increasing. Therefore, DAPK gene may participate in HL-60 cell apoptosis induced by 5-aza-2dC.

PMID:
24989283
[Indexed for MEDLINE]
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