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Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Jun;22(3):605-11. doi: 10.7534/j.issn.1009-2137.2014.03.006.

[IFN-γ stimulation enhances immunosuppressive capability of human umbilical cord mesenchymal stem cells].

[Article in Chinese]

Author information

  • 1Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China.
  • 2Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China; Cryomedical Laboratory, Shandong University Qilu Hospital, Jinan 250012, Shandong Province, China. E-mail:lidong73@sdu.edu.cn.

Abstract

This study was objective to explore the effect of IFN-γ on immunosuppressive capability of mesenchymal stem cells (MSC) derived from umbilical cord. The immunomodulating capability of MSC was changed by stimulating cell surface receptors like Toll-like receptors (TLR). The inhibition of T-lymphocyte proliferation by MSC was tested via cell co-cultures. Further RT-PCR and ELISA were performed to examine the expression changes in gene and protein level. The results showed that the IFN-γ could promote the immunosuppressive effect of umbilical cord derived MSC. IFN-γ-stimulated MSC could suppress the proliferation of T cells more effectively. IFN-γ stimulation up-regulated the expression of immunosuppressive genes like IDO1, COX2, HLA-G, and soluble suppressive proteins such as HLA-G, KYN, IL10, PGE2 of MSC. And the immuno suppression capability of IFN-γ-stimulated MSC was 2-7 folds higher than control in MSC and lymphocyte co-culture tests. It is concluded that IFN-γ can effectively enhance the immunosuppressive capability of MSC.

PMID:
24989262
[PubMed - indexed for MEDLINE]
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