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Plant Cell. 2014 Jul;26(7):2761-76. doi: 10.1105/tpc.114.128439. Epub 2014 Jul 2.

Evolution of the BBAA component of bread wheat during its history at the allohexaploid level.

Author information

1
Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, China.
2
Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.
3
Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, China Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011.
4
Key Laboratory for Applied Statistics of the Ministry of Education, Northeast Normal University, Changchun 130024, China.
5
Department of Life Sciences, Ben-Gurion University, Beer-Sheva 84105, Israel.
6
Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel.
7
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011.
8
Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, China baoliu@nenu.edu.cn.

Abstract

Subgenome integrity in bread wheat (Triticum aestivum; BBAADD) makes possible the extraction of its BBAA component to restitute a novel plant type. The availability of such a ploidy-reversed wheat (extracted tetraploid wheat [ETW]) provides a unique opportunity to address whether and to what extent the BBAA component of bread wheat has been modified in phenotype, karyotype, and gene expression during its evolutionary history at the allohexaploid level. We report here that ETW was anomalous in multiple phenotypic traits but maintained a stable karyotype. Microarray-based transcriptome profiling identified a large number of differentially expressed genes between ETW and natural tetraploid wheat (Triticum turgidum), and the ETW-downregulated genes were enriched for distinct Gene Ontology categories. Quantitative RT-PCR analysis showed that gene expression differences between ETW and a set of diverse durum wheat (T. turgidum subsp durum) cultivars were distinct from those characterizing tetraploid cultivars per se. Pyrosequencing revealed that the expression alterations may occur to either only one or both of the B and A homoeolog transcripts in ETW. A majority of the genes showed additive expression in a resynthesized allohexaploid wheat. Analysis of a synthetic allohexaploid wheat and diverse bread wheat cultivars revealed the rapid occurrence of expression changes to the BBAA subgenomes subsequent to allohexaploidization and their evolutionary persistence.

PMID:
24989045
PMCID:
PMC4145112
DOI:
10.1105/tpc.114.128439
[Indexed for MEDLINE]
Free PMC Article

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