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Plant Cell. 2014 Jul;26(7):3036-50. doi: 10.1105/tpc.114.126375. Epub 2014 Jul 2.

Combined increases in mitochondrial cooperation and oxygen photoreduction compensate for deficiency in cyclic electron flow in Chlamydomonas reinhardtii.

Author information

1
CEA, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, F-13108 Saint-Paul-lez-Durance, France CNRS, UMR 7265 Biologie Végétale et Microbiologie Environnementale, F-13108 Saint-Paul-lez-Durance, France Aix Marseille Université, UMR 7265 Biologie Végétale et Microbiologie Environnementale, F-13284 Marseille, France.
2
Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
3
CEA, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, F-13108 Saint-Paul-lez-Durance, France CNRS, UMR 7265 Biologie Végétale et Microbiologie Environnementale, F-13108 Saint-Paul-lez-Durance, France Aix Marseille Université, UMR 7265 Biologie Végétale et Microbiologie Environnementale, F-13284 Marseille, France gilles.peltier@cea.fr.

Abstract

During oxygenic photosynthesis, metabolic reactions of CO2 fixation require more ATP than is supplied by the linear electron flow operating from photosystem II to photosystem I (PSI). Different mechanisms, such as cyclic electron flow (CEF) around PSI, have been proposed to participate in reequilibrating the ATP/NADPH balance. To determine the contribution of CEF to microalgal biomass productivity, here, we studied photosynthesis and growth performances of a knockout Chlamydomonas reinhardtii mutant (pgrl1) deficient in PROTON GRADIENT REGULATION LIKE1 (PGRL1)-mediated CEF. Steady state biomass productivity of the pgrl1 mutant, measured in photobioreactors operated as turbidostats, was similar to its wild-type progenitor under a wide range of illumination and CO2 concentrations. Several changes were observed in pgrl1, including higher sensitivity of photosynthesis to mitochondrial inhibitors, increased light-dependent O2 uptake, and increased amounts of flavodiiron (FLV) proteins. We conclude that a combination of mitochondrial cooperation and oxygen photoreduction downstream of PSI (Mehler reactions) supplies extra ATP for photosynthesis in the pgrl1 mutant, resulting in normal biomass productivity under steady state conditions. The lower biomass productivity observed in the pgrl1 mutant in fluctuating light is attributed to an inability of compensation mechanisms to respond to a rapid increase in ATP demand.

PMID:
24989042
PMCID:
PMC4145130
DOI:
10.1105/tpc.114.126375
[Indexed for MEDLINE]
Free PMC Article

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