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Methods Mol Biol. 2014;1178:129-41. doi: 10.1007/978-1-4939-1016-8_12.

Human eosinophil adhesion and receptor expression.

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1
Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, 1111 Highland Avenue, WIMR II, Room 4528, Madison, WI, 53705, USA, cscurran@wisc.edu.

Abstract

Eosinophils migrate from the bone marrow in response to cytokines and chemokines which induce the expression and activation of adhesion receptors. In understanding the recruitment of eosinophils, protocols to identify eosinophil adhesion and receptor expression have been identified. In this summary, the eosinophil peroxidase and fluorescent labeling assays are described as measurements of indirect and direct eosinophil adhesion, respectively. Additional protocols that identify eosinophil receptor expression via immunofluorescent microscopy and flow cytometry are also described.

PMID:
24986613
DOI:
10.1007/978-1-4939-1016-8_12
[Indexed for MEDLINE]
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