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Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):E2866-74. doi: 10.1073/pnas.1410326111. Epub 2014 Jun 30.

Positron emission tomography probe demonstrates a striking concentration of ribose salvage in the liver.

Author information

1
Departments of Microbiology, Immunology, and Molecular Genetics, pclark@mednet.ucla.edu mphelps@mednet.ucla.edu owenwitte@mednet.ucla.edu.
2
Molecular and Medical Pharmacology,Crump Institute for Molecular Imaging.
3
Molecular and Medical Pharmacology,Chemistry and Biochemistry, and.
4
Molecular and Medical Pharmacology.
5
Departments of Microbiology, Immunology, and Molecular Genetics.
6
Division of Digestive Diseases.
7
Pasarow Mass Spectrometry Laboratory, Semel Institute for Neuroscience and Human Behavior,Psychiatry and Biobehavioral Sciences.
8
Molecular and Medical Pharmacology,Crump Institute for Molecular Imaging, pclark@mednet.ucla.edu mphelps@mednet.ucla.edu owenwitte@mednet.ucla.edu.
9
Chemistry and Biochemistry, and.
10
Departments of Microbiology, Immunology, and Molecular Genetics,Molecular and Medical Pharmacology,Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, andHoward Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 pclark@mednet.ucla.edu mphelps@mednet.ucla.edu owenwitte@mednet.ucla.edu.

Abstract

PET is a powerful technique for quantifying and visualizing biochemical pathways in vivo. Here, we develop and validate a novel PET probe, [(18)F]-2-deoxy-2-fluoroarabinose ([(18)F]DFA), for in vivo imaging of ribose salvage. DFA mimics ribose in vivo and accumulates in cells following phosphorylation by ribokinase and further metabolism by transketolase. We use [(18)F]DFA to show that ribose preferentially accumulates in the liver, suggesting a striking tissue specificity for ribose metabolism. We demonstrate that solute carrier family 2, member 2 (also known as GLUT2), a glucose transporter expressed in the liver, is one ribose transporter, but we do not know if others exist. [(18)F]DFA accumulation is attenuated in several mouse models of metabolic syndrome, suggesting an association between ribose salvage and glucose and lipid metabolism. These results describe a tool for studying ribose salvage and suggest that plasma ribose is preferentially metabolized in the liver.

KEYWORDS:

Slc2a2; molecular imaging; sugar metabolism

PMID:
24982199
PMCID:
PMC4104878
DOI:
10.1073/pnas.1410326111
[Indexed for MEDLINE]
Free PMC Article

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