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Mol Cell Biol. 2014 Sep 15;34(18):3407-20. doi: 10.1128/MCB.00106-14. Epub 2014 Jun 30.

MicroRNA 340 is involved in UVB-induced dendrite formation through the regulation of RhoA expression in melanocytes.

Author information

1
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
2
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China Department of Dermatology, Shaanxi Provincial Hospital of Traditional Chinese Medicine, Xi'an, China.
3
Department of Physiology, Fourth Military Medical University, Xi'an, China chxinli@fmu.edu.cn chengxinderm@163.com.
4
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China Department of Dermatology, Chinese People's Liberation Army General Hospital, Beijing, China chxinli@fmu.edu.cn chengxinderm@163.com.

Abstract

The influence of UV irradiation on pigmentation is well established, but the molecular and cellular mechanisms controlling dendrite formation remain incompletely understood. MicroRNAs (miRNAs) are a class of small RNAs that participate in various cellular processes by suppressing the expression of target mRNAs. In this study, we investigated the expression of miRNAs in response to UVB irradiation using a microarray screen and then identified potential mRNA targets for differentially expressed miRNAs among the genes governing dendrite formation. We subsequently determined the ability of miRNA 340 (miR-340) to suppress the expression of RhoA, which is a predicted miR-340 target gene that regulates dendrite formation. The overexpression of miR-340 promoted dendrite formation and melanosome transport, and the downregulation of miR-340 inhibited UVB-induced dendrite formation and melanosome transport. Moreover, a luciferase reporter assay demonstrated direct targeting of RhoA by miR-340 in the immortalized human melanocyte cell line Pig1. In conclusion, this study has established an miRNA associated with UVB irradiation. The significant downregulation of RhoA protein and mRNA expression after UVB irradiation and the modulation of miR-340 expression suggest a key role for miR-340 in regulating UVB-induced dendrite formation and melanosome transport.

PMID:
24980435
PMCID:
PMC4135612
DOI:
10.1128/MCB.00106-14
[Indexed for MEDLINE]
Free PMC Article
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