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Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9407-12. doi: 10.1073/pnas.1324147111. Epub 2014 Jun 16.

Recapitulation of the forward nuclear auxin response pathway in yeast.

Author information

1
Departments of Biology and.
2
Electrical Engineering, University of Washington, Seattle, WA 98195.
3
Departments of Biology and jn7@uw.edu klavins@uw.edu.
4
Electrical Engineering, University of Washington, Seattle, WA 98195 jn7@uw.edu klavins@uw.edu.

Abstract

Auxin influences nearly every aspect of plant biology through a simple signaling pathway; however, it remains unclear how much of the diversity in auxin effects is explained by variation in the core signaling components and which properties of these components may contribute to diversification in response dynamics. Here, we recapitulated the entire Arabidopsis thaliana forward nuclear auxin signal transduction pathway in Saccharomyces cerevisiae to test whether signaling module composition enables tuning of the dynamic response. Sensitivity analysis guided by a small mathematical model revealed the centrality of auxin/indole-3-acetic acid (Aux/IAA) transcriptional corepressors in controlling response dynamics and highlighted the strong influence of natural variation in Aux/IAA degradation rates on circuit performance. When the basic auxin response circuit was expanded to include multiple Aux/IAAs, we found that dominance relationships between coexpressed Aux/IAAs were sufficient to generate distinct response modules similar to those seen during plant development. Our work provides a new method for dissecting auxin signaling and demonstrates the key role of Aux/IAAs in tuning auxin response dynamics.

KEYWORDS:

signaling dynamics; synthetic biology

PMID:
24979769
PMCID:
PMC4084466
DOI:
10.1073/pnas.1324147111
[Indexed for MEDLINE]
Free PMC Article

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