Abstract
The nucleotide sequence of a 1689bp fragment of the Bacillus subtilis locus containing ptsX (a crr-like gene), ptsH (coding for HPr), and the 5'-end of ptsI (coding for Enzyme I) was determined. The deduced amino acid sequences of ptsH and the N-terminal part of ptsI were compared to those of Streptococcus faecalis and Escherichia coli. Transcription fusion demonstrated that ptsHI constitutes an operon. An open reading frame overlapping the main part of ptsH in the opposite sense was shown to be expressed in vivo, using protein fusions with beta-galactosidase. The deduced amino acid sequence of ptsX showed significant homology with that of Salmonella typhimurium glucose-specific Enzyme III. ptsX was preceded by an open reading frame whose amino acid sequence showed strong homology with the C-terminal part of E. coli Enzyme IIGlc.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / enzymology
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Bacillus subtilis / genetics*
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Bacterial Proteins*
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Base Sequence
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Cloning, Molecular
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Enterococcus faecalis / genetics
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Escherichia coli / genetics
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Genes, Bacterial*
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Molecular Sequence Data
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Multigene Family
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Operon
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Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
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Phosphotransferases (Nitrogenous Group Acceptor)*
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Plasmids
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Recombinant Fusion Proteins / biosynthesis
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Restriction Mapping
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Staphylococcus aureus / genetics
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beta-Galactosidase / genetics
Substances
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Bacterial Proteins
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Recombinant Fusion Proteins
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Phosphoenolpyruvate Sugar Phosphotransferase System
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phosphocarrier protein HPr
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Phosphotransferases (Nitrogenous Group Acceptor)
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phosphoenolpyruvate-protein phosphotransferase
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beta-Galactosidase