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Cell Calcium. 2014 Aug;56(2):96-107. doi: 10.1016/j.ceca.2014.05.004. Epub 2014 Jun 6.

Characterization of ryanodine receptor type 1 single channel activity using "on-nucleus" patch clamp.

Author information

1
Department of Pharmacology and Physiology, University of Rochester, 601 Elmwood Ave, Rochester, NY 14642, United States.
2
Department of Pharmacology and Physiology, University of Rochester, 601 Elmwood Ave, Rochester, NY 14642, United States. Electronic address: David_Yule@urmc.rochester.edu.

Abstract

In this study, we provide the first description of the biophysical and pharmacological properties of ryanodine receptor type 1 (RyR1) expressed in a native membrane using the on-nucleus configuration of the patch clamp technique. A stable cell line expressing rabbit RyR1 was established (HEK-RyR1) using the FLP-in 293 cell system. In contrast to untransfected cells, RyR1 expression was readily demonstrated by immunoblotting and immunocytochemistry in HEK-RyR1 cells. In addition, the RyR1 agonists 4-CMC and caffeine activated Ca(2+) release that was inhibited by high concentrations of ryanodine. On nucleus patch clamp was performed in nuclei prepared from HEK-RyR1 cells. Raising the [Ca(2+)] in the patch pipette resulted in the appearance of a large conductance cation channel with well resolved kinetics and the absence of prominent subconductance states. Current versus voltage relationships were ohmic and revealed a chord conductance of ∼750pS or 450pS in symmetrical 250mM KCl or CsCl, respectively. The channel activity was markedly enhanced by caffeine and exposure to ryanodine resulted in the appearance of a subconductance state with a conductance ∼40% of the full channel opening with a Po near unity. In total, these properties are entirely consistent with RyR1 channel activity. Exposure of RyR1 channels to cyclic ADP ribose (cADPr), nicotinic acid adenine dinucleotide phosphate (NAADP) or dantrolene did not alter the single channel activity stimulated by Ca(2+), and thus, it is unlikely these molecules directly modulate RyR1 channel activity. In summary, we describe an experimental platform to monitor the single channel properties of RyR channels. We envision that this system will be influential in characterizing disease-associated RyR mutations and the molecular determinants of RyR channel modulation.

KEYWORDS:

Inositol 1,4,5-trisphosphate receptor; Ryanodine receptor; Single channel measurement

PMID:
24972488
PMCID:
PMC4120733
DOI:
10.1016/j.ceca.2014.05.004
[Indexed for MEDLINE]
Free PMC Article

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