Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biomed Opt. 2014 Jun;19(6):060503. doi: 10.1117/1.JBO.19.6.060503.

Two-step phase-shifting fluorescence incoherent holographic microscopy.

Author information

1
Clemson University, Department of Bioengineering and COMSET, Clemson, South Carolina 29634.
2
Shenzhen University, Institute of Optoelectronics, Shenzhen, Guangdong 518060, China.
3
Tianjin University, State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin 300072, China.

Abstract

Fluorescence holographic microscope (FINCHSCOPE) is a motionless fluorescence holographic imaging technique based on Fresnel incoherent correlation holography (FINCH) that shows promise in reconstructing three-dimensional fluorescence images of biological specimens with three holograms. We report a developing two-step phase-shifting method that reduces the required number of holograms from three to two. Using this method, we resolved microscopic fluorescent beads that were three-dimensionally distributed at different depths with two interferograms captured by a CCD camera. The method enables the FINCHSCOPE to work in conjunction with the frame-straddling technique and significantly enhance imaging speed.

PMID:
24972355
PMCID:
PMC4073681
DOI:
10.1117/1.JBO.19.6.060503
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems Icon for PubMed Central
    Loading ...
    Support Center