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PLoS One. 2014 Jun 27;9(6):e99367. doi: 10.1371/journal.pone.0099367. eCollection 2014.

Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.

Author information

1
Unité mixte de recherche Amélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales, Centre International de Recherche Agronomique pour le Développement, Montpellier, France; Department of Agriculture, Rubber Research Institute, Bangkok, Thailand.
2
Beijing Institute of Genomics, Chinese Academy of Science, Beijing, China.
3
Unité mixte de recherche Amélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales, Centre International de Recherche Agronomique pour le Développement, Montpellier, France; Indonesian Biotechnology Research Institute for Estate Crops, Bogor, Indonesia.
4
Unité mixte de recherche Amélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales, Centre International de Recherche Agronomique pour le Développement, Montpellier, France.
5
Department of Agriculture, Rubber Research Institute, Bangkok, Thailand.
6
Sembawa Research Center, Indonesian Rubber Research Institute, Palembang, Indonesia.

Abstract

The AP2/ERF superfamily encodes transcription factors that play a key role in plant development and responses to abiotic and biotic stress. In Hevea brasiliensis, ERF genes have been identified by RNA sequencing. This study set out to validate the number of HbERF genes, and identify ERF genes involved in the regulation of latex cell metabolism. A comprehensive Hevea transcriptome was improved using additional RNA reads from reproductive tissues. Newly assembled contigs were annotated in the Gene Ontology database and were assigned to 3 main categories. The AP2/ERF superfamily is the third most represented compared with other transcription factor families. A comparison with genomic scaffolds led to an estimation of 114 AP2/ERF genes and 1 soloist in Hevea brasiliensis. Based on a phylogenetic analysis, functions were predicted for 26 HbERF genes. A relative transcript abundance analysis was performed by real-time RT-PCR in various tissues. Transcripts of ERFs from group I and VIII were very abundant in all tissues while those of group VII were highly accumulated in latex cells. Seven of the thirty-five ERF expression marker genes were highly expressed in latex. Subcellular localization and transactivation analyses suggested that HbERF-VII candidate genes encoded functional transcription factors.

PMID:
24971876
PMCID:
PMC4074046
DOI:
10.1371/journal.pone.0099367
[Indexed for MEDLINE]
Free PMC Article

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