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Front Chem. 2014 Jun 12;2:38. doi: 10.3389/fchem.2014.00038. eCollection 2014.

(1)H high resolution magic-angle coil spinning (HR-MACS) μNMR metabolic profiling of whole Saccharomyces cervisiae cells: a demonstrative study.

Author information

1
CEA Saclay, DSM, IRAMIS, UMR CEA/CNRS 3299 - NIMBE, Laboratoire Structure et Dynamique par Résonance Magnétique Gif-sur-Yvette, France.
2
Department of Chemistry, University of York Heslington, York, UK.

Abstract

The low sensitivity and thus need for large sample volume is one of the major drawbacks of Nuclear Magnetic Resonance (NMR) spectroscopy. This is especially problematic for performing rich metabolic profiling of scarce samples such as whole cells or living organisms. This study evaluates a (1)H HR-MAS approach for metabolic profiling of small volumes (250 nl) of whole cells. We have applied an emerging micro-NMR technology, high-resolution magic-angle coil spinning (HR-MACS), to study whole Saccharomyces cervisiae cells. We find that high-resolution high-sensitivity spectra can be obtained with only 19 million cells and, as a demonstration of the metabolic profiling potential, we perform two independent metabolomics studies identifying the significant metabolites associated with osmotic stress and aging.

KEYWORDS:

HR-MACS; Metabolic Profiling; Saccharomyces cervisiae; cell growth; micro-NMR; osmotic stress

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